In the present study, a majority of cells in the FOXO3-high gastric cancer cell lines were FOXO3-Cyt cells, with FOXO3-Nuc cells included as a minor population, suggesting that shuttling FOXO3 between the cytoplasm and nucleus, and nuclear-localized FOXO3 in a minor population plays other important roles. Here, FOXO3 is linked to gastric cancer.