To verify the metastatic activity of HOXC6, lentivirus methods were used to construct HOXC6 overexpression (HOXC6-OE) and knockdown (HOXC6-KD) cell lines in HCT116 and RKO cells because the expression of HOXC6 was high in RKO cells and low in HCT116 cells based on testing six common CRC cell lines (Fig. 2A–C). This evidence concerns the gene HOXC6 and colorectal carcinoma.