To examine if the non-conservative Gly316Asp amino acid replacement altered the stability or function of MLKL in human cells, we examined this mutant relative to wild-type MLKL in two cell lines commonly used to study necroptotic signal transduction: the HT29 colon adenocarcinoma and U937 monocytic cell lines. This evidence concerns the gene MLKL and colon adenocarcinoma.