To validate the results obtained with the lung cancer xenograft, showing that their proliferation is highly dependent on SMAC and study SMAC mode of action, we generated CRISPR/Cas9‐mediated SMAC knockout (SMAC‐KO) A549 lung cancer (A549:SMACΔ/Δ) and HEK‐T‐293 (HEK:SMACΔ/Δ) noncancerous cell lines )Fig. 2A). Here, DIABLO is linked to lung carcinoma.