To this end, we performed gene upregulation studies, immunotherapy assay, dual luciferase assay, and immunoprecipitation assay to reveal (1) the relationship between hsa-miR-128a and the p53 pathway in laryngeal squamous cell carcinoma and (2) the effect of hsa-miR-128a upregulation on the pembrolizumab sensitivity of laryngeal cancer cells. The gene discussed is TP53; the disease is laryngeal squamous cell carcinoma.