Knockdown of YTHDF1 repressed GC cell growth and metastasis in vitro and in vivo. Intersection co-analysis for RNA-seq, Methylated RNA immune-precipitation (MeRIP)-seq/qPCR, Co-immunoprecipitation (Co-IP), RNA immunoprecipitation (RIP)-qPCR, luciferase analysis and RNA pull-down revealed that YTHDF1 facilitated USP14 translation via a m6A-dependent way, and USP14 upregulation harbored a positive correlation with YTHDF1 expression and indicated a poor prognosis in GC. This evidence concerns the gene USP14 and gastric cancer.