Because CYP24A1 was demonstrated as a targeting protein of miR-1278, we investigated whether overexpression of miR-1278 facilitates CRC cell death after treatment with vitamin D. The IC50 of vitamin D decreased to 0.58 and 1.26 μM in the miR-1278 mimic group compared to 1.06 and 1.97 μM in the control group in HT29 and HCT116 cells, respectively (Figures 6A,B). The gene discussed is CYP24A1; the disease is colorectal carcinoma.