To elucidate the functional relationship between KDM2B and EZH2 in CRC, we selectively silenced the KDM2B gene via the lentiviral expression vector of short hairpin RNA (shRNA) against KDM2B (shKDM2B) and negative control (NC) in HT-29 and DLD-1 cells. The gene discussed is KDM2B; the disease is colorectal carcinoma.