GO and KEGG enrichment analyses suggested that IL-6 intervention before osteosarcoma cell treatment with lobaplatin significantly regulated Rho GTPase binding proteins and the tubulin complex as well as the estrogen signaling pathway, apoptosis pathway, and IL-17 signaling pathway, which is partly in accordance with our PPI analysis and the flow cytometry and Hoechst staining results. This evidence concerns the gene IL17A and osteosarcoma.