In this work, we found that the crosstalk between cancer cells and BMFs remarkably upregulated the PD-L1 expression in BMFs by activation the Wnt/β-catenin signaling, meanwhile the inhibition of Wnt/β-catenin signaling pathway can significantly suppress the PD-L1 expression in BMFs, Despite the use of ICBs has dramatically increased the survival periods of tumor patients, the response rate was still relatively low in most of the cancer types. The gene discussed is CD274; the disease is cancer.