In another study, Misra et al. generated a p53-EGFP-C3 fusion construct which expressed GFP to allow an estimation of p53 mediated anti-glioma activity and delivered them to glioma cells through a cationic cholesterol based nanocarrier prepared by mixing cationic cholesterol Gemini (ChoL-5L) with natural lipid DOPE in a molar 1:4 ratio. The gene discussed is TP53; the disease is central nervous system cancer.