IFNA1 and systemic lupus erythematosus: However, when the IFN-High CD8+ T cells were cultured in vitro they lost their IFN signature very quickly and by day 3 the ISM was equivalent to the one observed in IFN-Neg cells (Supplementary Fig. 9c), a change that prevented us from pursuing further in vitro functional assays with the cells from the SLE patients.