We verified the expression level of FASN in our PCSK9-overexpressing and PCSK9-downregulated cell lines with western blotting and found that overexpression of PCSK9 promoted the expression of FASN in HCC cell lines (p = 0.001) (Fig. 5a) and that downregulation of PCSK9 reduced the expression levels of FASN (p = 0.008) (Fig. 5b). This evidence concerns the gene FASN and hepatocellular carcinoma.