We then performed a t-Distributed Stochastic Neighbor Embedding (tSNE) analysis on a panel including the two monocyte phenotypic markers (CD14 and CD16) besides C9 and FLICA, where TB-IRIS and TB non-IRIS monocytes were directly compared and clustered against each other (for more details, please refer to the method section). The gene discussed is C9; the disease is tuberculosis.