To investigate if antitumor responses are associated with the production of inflammatory soluble factors, the levels of chemokines and inflammatory cytokines (IL10, IL6, IL12p70, TNF, IFNγ, MCP-1, TGFß and CXCL10) were investigated in mouse plasma of RM-1 tumor bearing mice after 2 weeks of treatment with vehicle, GPB730, anti-CTLA-4 or anti-CTLA-4 + GP730 and in tumor naïve mice (Fig. 6). This evidence concerns the gene TNF and neoplasm.