In this study, using VEGFR‐3 knockdown (VEGFR‐3f/−) mice or wild‐type (WT) mice treated with a recombinant human VEGF‐C156S mutant, we examined the potential role of VEGF‐C‐VEGFR‐3 signaling in cardiac hypertrophy and dysfunction induced by transverse aortic constriction (TAC). Here, VEGFC is linked to cardiac hypertrophy.