To investigate the function of MTSS1, a gene knockout was performed in the human AML cell line TF-1 using two sgRNAs, both of which target the first exon of MTSS1 downstream of the ATG codon (sgMTSS1_1, sgMTSS1_2; Supplementary Fig. S3a). The gene discussed is TF; the disease is acute myeloid leukemia.