In addition, the activated human effector T cell induced more severe cell death in MTHFD2-KO SW1990 cells (Fig. 1d and Supplementary Fig. 1f), and an antigen-specific cytotoxicity assay between activated splenocytes from OT-1 mice and mouse pancreatic cancer OVA-Pan02 cells also showed an increased cell death in MTHFD2-KD Pan02 cells (Fig. 1e and Supplementary Fig. 1g). This evidence concerns the gene MTHFD2 and pancreatic neoplasm.