MTHFD2 and liver cancer: We therefore analyzed T cell cytotoxicity in kidney cancer 786-O cells, lung cancer A549 cells, breast cancer MCF-7 cells, liver cancer HepG2 cells, and colon cancer HCT-116 cells using MTHFD2 siRNA (Supplementary Fig. 1h) and obtained results similar to those in SW1990 cells (Fig. 1c) for all these cancer cell lines (Fig. 1g and Supplementary Fig. 1i).