JAG1 and osteosarcoma: Immunohistochemical results indicated that Jagged1 expression was strongly positive in 69% osteosarcoma samples (47/68), it was more noteworthy that in 24 metastatic samples, the positive stain is 87.5% including 17 strong positive cases (+++), 4 moderate positive cases (++), whereas among non-metastatic samples, the positive stain is 59% (26/44), including 6 strong positive cases (+++) and 20 moderate positive cases (++).