In total, 116 patients with suspected optic neuropathy were examined, and 25 cases of LHON were diagnosed (Fig. 1).The diagnostic rate for LHON of the Part A was 21.6% in our optic atrophy group using the mtDNA Sanger sequencing panel (OPA1, WFS1, etc., not included). Here, WFS1 is linked to Optic neuropathy.