In our study, upregulated NEU1 offset the effects of miR-23b-3p on body weight, fat percent, FBG, FI and AUC of both OGTT and ITT, and inflammatory factors (CRP, IL-6, TNF-α, MCP1) in mice with obesity-induced IR. This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.