As expected, it was observed that PI3K, AKT, GSK3β, mTOR, P70S6K, and eIF-4E were significantly phosphorylated in the TAC group, suggesting that PI3K-Akt signaling pathway was indeed activated in cardiac hypertrophy (Figures 3(d) and 3(e)). Here, GSK3B is linked to cardiac hypertrophy.