SPN and pneumococcal infection: To achieve a comprehensive understanding of inflammation-related genes modulated by NanA and Siglec-5 in response to pneumococcal infection, we infected Siglec-5 overexpressing THP-1 cells (Sig-5/THP-1) with wild-type (WT) SPN or isogenic sialidase deficient mutant (ΔnanA) and profiled the expression of 579 immune‐related genes using the NanoString nCounter Human Immunology Panel.