Later, we assessed the colony formation of our cell lines using soft agar assay, which is generally employed to confirm the transformation ability of the cells; we noted a significant increase in the number and size of colonies for both breast cancer cell lines, MCF7 and MDA-MB-231, expressing E6/E7 and LMP1 together (3.8-fold and 7.6-fold) compared to E6/E7 (0.8-fold and 1.2-fold) and LMP1 (1.4-fold and 3.2-fold) alone and their matched controls in MCF7 and MDA-MB-231, respectively as shown in Figures 4A,B. This evidence concerns the gene PDLIM7 and breast cancer.