Interestingly, IFN-γ was cytokine which had significantly different levels between vaccinated and unvaccinated mice at day 14 post-cyst-challenge, suggesting that IFN-γ is also central to the development of immunity against T. gondii cyst infection, consistent with Δgra9-vaccination-induced immune response for protection of cyst challenge. The gene discussed is IFNG; the disease is cyst.