Similarly, in serum, alanine aminotransferase (ALT) level of WD mice was about two folds of CD (306.1 ± 68.8 vs. 163.5 ± 46.0; p < 0.001) and TNF-α level showed significantly higher than CD (34.1 ± 1.4 vs. 30.5 ± 1.9; p < 0.05), though the differences of ALT and TNF-α between CD and CD+7KC or WD and WD+7KC could not be observed (Figure 1D). This evidence concerns the gene TNF and Wilson disease.