Furthermore, in the in vivo conditions, the effective concentration of ghrelin was 25 μg/kg in the TNBS model and 125 μg/kg in the DSS model, consistent with the previous studies on the application of ghrelin in colitis models induced by TNBS and DSS (Gonzalez-Rey et al., 2006; Konturek et al., 2009). The gene discussed is GHRL; the disease is colitis.