1) MIF levels were increased in serum (peak at 9 h: 197 ± 9 ng/ml), ascitic fluid and lung, but not in pancreas or liver in AP model; 2) Anti-MIF Ab reduced lung TNF-α levels and improved survival rate (88 vs. 44%, given 1 h before; 92 vs. 33%, given immediately; 61 vs. 39%, given 1 h after induction) of AP rats. The gene discussed is MIF; the disease is alkaline phosphatase measurement.