1) MIF-induced increase in miR-301b targets and reduces NR3C2 levels in PANC-1 and Capan-2 cell lines; 2) NR3C2 inhibited proliferation, colony formation, migration, invasion, and enhances sensitivity of PC cell lines to gemcitabine; 3) MIF enhanced PC cell lines invasiveness by targeting NR3C2 through the upregulation of miR-301b; 4) AZD6244 didn’t alter miR-301b or NR3C2 in MIF-overexpressing PC cell lines; 5) treatment of MIF-overexpression PC cells with LY294002 resulted in miR-301b decrease and NR3C2 increase. Here, MIF is linked to pachyonychia congenita.