MIF and alkaline phosphatase measurement: Circulating MIF levels are significantly higher in obese or type 2 diabetic populations compared to healthy controls (Morrison and Kleemann, 2015), are significantly elevated in experimental and human AP and correlated with disease severity (Sakai et al., 2003), and are also highly up-regulated in exosomes (Costa-Silva et al., 2015) and in PDAC tissue (Funamizu et al., 2013; Lippitz, 2013; Tan et al., 2014).