To determine the mechanism by which let-7i-5p regulated HABP4 expression through the HABP4 3′-UTR binding site using bioinformatics analysis, pmirGLO-WT-HABP4 and pmirGLO-MUT-HABP4 plasmids were synthesized, after which a HABP4 3′-UTR luciferase reporter assay was conducted in ccRCC cells (Fig. 7a). Here, HABP4 is linked to nonpapillary renal cell carcinoma.