Of the three CpG dinucleotides, CpG1 was mainly associated with the expression of MYBPHL in both HMCLs and primary samples of MM, therefore, these data not only rekindled the role of single-site CpG methylation in the regulation of gene expression, but also demonstrated that regulation by single-site CpG methylation was not restricted to the promoter region28,29. This evidence concerns the gene MYBPHL and Miyoshi myopathy.