The SURF1 group included (i) SURF1_Mut lines that we derived from LS patients (S1, S2) and (ii) CTL_Mut lines that we obtained by introducing the c.769G>A mutation into both alleles of control C1 iPSCs (lines C1_Mut1, C1_Mut2) (Fig. 1d). Here, SURF1 is linked to Leigh syndrome.