And we also detected the expression of PARP and caspase-3 by western blot after adding different concentrations of anisomycin, and found that breast cancer cells with higher concentration anisomycin showed significantly increased expression of both cleaved caspase-3 and cleaved PARP than those with lower concentration anisomycin (see Figures 6A–E). Here, CASP3 is linked to breast carcinoma.