Furthermore, a significantly different IHC staining of p-ERK was observed in GPER-positive (72/100, 72.0%) and GPER-negative (12/41, 29.3%) HCC tissue (P < 0.0001, Figure 5B), indicating that the GPER/ERK pathway was strongly associated with GPER-positive patients. The gene discussed is GPER1; the disease is hepatocellular carcinoma.