NEDD4 and hepatocellular carcinoma: To determine whether the interplay of NEDD4 and HBx attenuates HBV-associated HCC progression, we upregulated HBx expression in NEDD4-overexpressing cells (OE-HBx+OE-NEDD4) and generated HBx-overexpressing cells (OE-HBx) as a control group (Figure 4C).