Additionally, analysis of TCGA RNA-seq data in TIMER database, we found consistent results, i.e. the expression of Nuf2 in HCC was significantly higher than that in normal tissue, as well as other 16 tumor tissues (Figure 1B), suggesting that Nuf2 may play a role in tumorigenesis, especially in HCC, and has the potential to be a diagnostic gene for liver cancer and other cancers. This evidence concerns the gene NUF2 and liver cancer.