To inquire prognostic biological markers of HCC, we applied the GEPIA to analyze the influence of hub genes expression level on survival of HCC patients and found that, except CCNB2, the high level gene expression of CDC20, CDK1, MAD2L1, BUB1, BUB1B, CCNB1, and CCNA2 were related to HCC patients poor overall survival. This evidence concerns the gene BUB1 and hepatocellular carcinoma.