TLR4 and infection: We orally infected WT and CLEC18A-2xHA transgenic mosquitoes using infected BMs, and used qPCR to test for differences in gene expression levels (using RPS7 as a reference) for several components of the Toll, IMD, JAK/STAT, and RNAi immune response pathways at several different time points: pre-BM, and 1, 3, and 7 days post-infection, to facilitate comparisons with the viral load experiment.