U-CH1 cells were stably co-transfected with si-NONHSAT024778/Lv-Robo1 or Lv-NONHSAT024778/si-Robo1 and control vector and then were subcutaneously injected into NOD/SCID mice separately to further confirm the effects of NONHSAT024778/miR-1290/Robo1 on chordoma cell growth in vivo. Here, ROBO1 is linked to chordoma.