The focus of this paper is directed at the large-scale preparation of sMB-Rs that would allow us to undertake their biophysical and functional characterisation, to ask whether in vitro release of MKLP1 from LIM1863 CRC cells is a general phenomenon or a cancer-cell specific process, and whether MKLP1 is an indicative marker of sMB-Rs. The gene discussed is KIF23; the disease is colorectal carcinoma.