The two genes with the smallest p-values (p-value < 10–3) (Table 1): BANP, p-value = 7.23 × 10–4 and PDCD6IP, p-value = 8.36 × 10–4, both downregulated in cases; and the two most relevant mRNAs associated with ischemic neuronal death from the top fifteen most significant differentially expressed mRNAs: CAMK2G, downregulated in cases (p-value = 4.52 × 10–3), and E2F4, upregulated in cases (p-value = 4.77 × 10–3), were selected for a second study using qRT-PCR in a new cohort of ten CADASIL patients and eight controls. This evidence concerns the gene E2F4 and CADASIL.