ALYREF protein expression levels were high in MYCN-amplified and MYCN overexpressing human neuroblastoma cells (lanes 1–7) across a panel of neuroblastoma cell lines (Fig. 2b–c), with significantly higher expression levels when compared to human fibroblasts (lanes 12 and 13; WI-38 and MRC5; Fig. 2b, c). Here, MYCN is linked to neuroblastoma.