Silencing endogenous ALYREF with ALYREF siRNA-1 or ALYREF siRNA-2 (lanes 2–3 and 5–6, Fig. 3a) in MYCN-amplified neuroblastoma cells significantly decreased MYCN protein expression (Fig. 3a), however, ALYREF knockdown had no effect on MYCN mRNA levels (Fig. 3b). Here, MYCN is linked to neuroblastoma.