ALYREF and neuroblastoma: By further examining pan-cancer cell line gene expression (RNA-seq) and copy number profiles using Cancer Cell Line Encyclopedia (CCLE)/Cancer Dependency Map33, we observed that neuroblastoma cell lines had the highest ALYREF gene expression and copy number compared with all other cancer types, supporting the principle that ALYREF is an abundant gene target in neuroblastoma (Supplementary Fig. 1g, h).