To investigate whether USP3 is responsible for the ALYREF-induced increase in MYCN stability and neuroblastoma cell growth, we created a pool of clones of Kelly and SK-N-BE(2)C cells (Fig. 7e) stably overexpressing either Vector control (Vector; lane 2) or USP3 (USP3; lane 3), and evaluated the effect of USP3 overexpression on MYCN ubiquitination and neuroblastoma cell viability following ALYREF knockdown. Here, MYCN is linked to neuroblastoma.