Further analysis for both overall (Fig. 6c) and event-free (Supplementary Fig. 6c) survival showed that MYCN-amplification and ALYREF expression had the greatest negative effect on USP3’s prognostic significance, suggesting that the role of USP3 in the malignant neuroblastoma cell phenotype is dependent on the MYCN-ALYREF regulatory complex. Here, USP3 is linked to neuroblastoma.