To further examine ALYREF as a transcriptional coactivator for MYCN, we evaluated the effect of ALYREF knockdown on USP3 gene transcription in SHEPMYCN3 neuroblastoma cells, which express MYCN under the control of doxycycline, therefore minimising ALYREF knockdown effects on MYCN stability. The gene discussed is MYCN; the disease is neuroblastoma.