To directly address the role of TAMs in PVSRIPO/PRR agonist-induced cytokine responses, we generated single-cell suspensions from GBM samples, depleted CD14+ cells (Supplementary Fig. 4a), and treated mock- and CD14+-depleted suspensions with PVSRIPO, LPS, Poly(I:C), or cGAMP. This evidence concerns the gene CD14 and glioblastoma.