To test whether ASAH1 is necessary for tumor growth, we knocked down ASAH1 expression in multiple melanoma cell lines (A375, M14, MeWo, and YUGASP) using shRNAs (Supplementary Figs. 3A and 3B) and evaluated anchorage-independent growth using a soft-agar assay. This evidence concerns the gene ASAH1 and neoplasm.