However, DDX3X deficiency did not restore NLRP3 activation after IAV–ΔNS1 infection (Fig. S3B), and we instead observed that CASP1 cleavage was reduced in Ddx3xfl/flLysMCre BMDMs compared with Ddx3xfl/fl BMDMs when infected with IAV (Fig. 3C, Fig. S3B). This evidence concerns the gene NLRP3 and infection.