Here, we aimed to study the role of DNA methylation in AKI by analysing the function of Methyl‐CpG–binding domain protein 2 (MBD2), a key methylated protein reader, in RM‐induced AKI, both in vitro using a mouse proximal tubule‐derived cell line (BUMPT) and in vivo using C57BL/6 mice. Here, MBD2 is linked to acute kidney injury.