We also detected other neutrophil activation markers such as CD11b and CD16 [28] on peripheral, intratumoral, peritumoral, and nontumor tissue neutrophils, and found that intratumoral neutrophils from GC patients expressed a significantly higher level of CD11b and CD16 than those expressed on peritumoral and nontumor tissue neutrophils; however, peripheral neutrophils expressed little CD11b and CD16 (Supplementary Figure 2). This evidence concerns the gene ITGAM and gastric cancer.