LEP and breast carcinoma: As shown in Figure 4 and Supplementary Figure 2, leptin increased migration in a scratch-wound assay in both cell lines, and pharmacological inhibition of autophagy with CQ decreased migration at 24 and 48 h in MDA-MB-231 (Figure 4C) and at 48 h in MCF7 cells (Figure 4B), suggesting that autophagy plays an important role in leptin-induced migration in breast cancer cells.