In the present study, TLR4-defective C3H/HeJ mice and wild-type C3H/HeN mice were intramuscularly injected with C. perfringens type A. As shown in Figure 1A, about half of C3H/HeN mice (24/45) died three days after C. perfringens infection, whereas 80% of C3H/HeJ mice (36/45) died at the same time. This evidence concerns the gene TLR4 and infection due to clostridium perfringens.