We present evidence, herein, that a novel and stable formulation of a RA and TGFβ-formulated single microparticle, decorated with a T1D-relevant autoantigen (Insulin B9-23 peptide) (41–44) can prevent the onset of hyperglycemia when administered into NOD mice that are at the mid-stage of active islet-selective autoimmunity. Here, TGFB1 is linked to Hyperglycemia.