To further confirm whether AMPK activation mediated the protective effects of FGF1ΔHBS against DCM, wild type (WT) and AMPKα2 (the main cardiac catalytic isoform27,28) knockout mice (AMPKα2−/−) (Supplemental Fig. S6a) were subjected to the DCM model established by HFD feeding plus STZ injection. Here, PRKAA2 is linked to familial dilated cardiomyopathy.